Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: List of antibodies

Article Snippet: mAb Mouse anti-human MMP2 (#436,000) , // , 1:300 , // , Thermo Fisher Scientific, MA, USA.

Techniques:

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: In HTR-8/SVneo cells, CNTF treatment induced MMP-1 downregulation and MMP-9 upregulation. Left, Western blots ( a – e ); right, histograms ( a ’– e ’) showing band quantification. a , a ’, b , b ’, c , c ’ HTR-8/SVneo cells and d , d ’, e , e ’ BeWo cells after treatment with 50 ng/ml rhCNTF. a ’ Significant MMP-1 downregulation; b ’ lack of significant differences in MMP-2; c ’ significant MMP-9 upregulation; d ’ lack of significant differences in MMP-2 protein levels; e ’ unchanged MMP-9 protein expression. MMP-1, MMP-2 and MMP-9 quantities were normalized using β‐actin. Results are expressed in arbitrary units (A.U.) and reported as histogram bars. Data are mean ± SD ( n = 3). * p < 0.05

Article Snippet: mAb Mouse anti-human MMP2 (#436,000) , // , 1:300 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Western Blot, Expressing

Journal: Cell Reports Medicine

Article Title: Mechanical confinement promotes heat resistance of hepatocellular carcinoma via SP1/IL4I1/AHR axis

doi: 10.1016/j.xcrm.2023.101128

Figure Lengend Snippet: 3D thermal ablation model (A) Orthotopic HCC tumor after thermal ablation for 30 s in BALB/C nude mice. Cell viability determined by NADH staining in the non-lethal, sublethal, and lethal regions of tumor tissue after thermal ablation for 30 s. (B) A long cuboid construct (25∗10∗0.8 mm 3 ) for thermal ablation (left) monitored by infrared imaging (right). (C) Live/dead staining of MHCC-97H in the different regions of the 3D model after thermal ablation. (D) The distance from the lethal to the non-lethal region in 3D and animal models (five biological replicates). (E) Schematic diagram of a hyperthermal device (left) and its infrared imaging during operation for 15 min (right). (F and G) Live/dead staining of MHCC-97H and quantifications in the different regions under temperature gradients generated by the homemade hyperthermal device for 15 min (three biological replicates). (H) The bright field images and live/dead staining of spheroids (red arrows) in the 3D model with or without 49°C treatment for 15 min. (I) Immunofluorescence on the 3D model with or without 49°C treatment for 15 min and the animal thermal ablation model for N-cadherin (red), E-cadherin (red), F-Actin (green), and Hoechst (blue). (J) Immunohistochemistry staining of N-cadherin and E-cadherin from the animal thermal ablation model. (K) E-cadherin, N-cadherin, and MMP2 protein expression detected by western blot assay in the 3D model after different heat treatments. Mean ± standard deviation. Statistical analysis, two-tailed Student’s t test for (D), one-way ANOVA with post Tukey’s comparisons test for (G). n.s., not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Mouse anti-human MMP2 , Proteintech , Cat#66366-1-Ig; RRID: AB_2881746.

Techniques: Staining, Construct, Imaging, Generated, Immunofluorescence, Immunohistochemistry, Expressing, Western Blot, Standard Deviation, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Mechanical confinement promotes heat resistance of hepatocellular carcinoma via SP1/IL4I1/AHR axis

doi: 10.1016/j.xcrm.2023.101128

Figure Lengend Snippet:

Article Snippet: Mouse anti-human MMP2 , Proteintech , Cat#66366-1-Ig; RRID: AB_2881746.

Techniques: Virus, Recombinant, Saline, Bicinchoninic Acid Protein Assay, Imaging, Staining, Transfection, Software, Protein-Protein interactions, Microscopy, Fluorescence, Liquid Chromatography, Mass Spectrometry

Journal: Cellular and Molecular Life Sciences

Article Title: TRP channel expression correlates with the epithelial–mesenchymal transition and high-risk endometrial carcinoma

doi: 10.1007/s00018-021-04023-1

Figure Lengend Snippet: TRP channels in primary EC biopsies: A Quantitative RT-qPCR showing TRP channel mRNA expression (TRPV2, TRPV4, TRPV6, TRPC1, TRPC4, TRPC6, TRPM4 and TRPM7) in EC cancer biopsies. Results are shown as relative expression to the geometric mean of housekeeping genes HPRT, PGK, ACTB and GAPDH. Colors indicate association with the epithelial phenotype (blue), the mesenchymal phenotype (red) or both (pink) in healthy endometrial cells. Data are represented as mean ± SEM. N = 54 p.s. B Pearson correlation coefficients between TRP channels and epithelial (CDH1, EPCAM, KRT18, MMP7) and mesenchymal (CDH2, ACTA2, MMP2, MMP9, ZEB1 and CTSB) marker genes in primary EC biopsies. Red indicates a positive correlation, while blue indicates a negative correlation. ** p < 0.01. N = 54 p.s

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-human E-cadherin (1/30, Abcam), rabbit monoclonal anti-human vimentin (1/100, Abcam), mouse monoclonal anti-human cytokeratin (1/100 Agilent), mouse monoclonal anti-human MMP2 (1/100, Abcam), and mouse monoclonal anti-human α-smooth muscle actin (1/100, Agilent).

Techniques: Quantitative RT-PCR, Expressing, Marker

Journal: Cellular and Molecular Life Sciences

Article Title: TRP channel expression correlates with the epithelial–mesenchymal transition and high-risk endometrial carcinoma

doi: 10.1007/s00018-021-04023-1

Figure Lengend Snippet: TRP channels in metastatic EC biopsies: A , C Heat maps of mRNA expression of epithelial (CDH1, KRT18, EPCAM, MMP7) and mesenchymal (MMP2, MMP9, CTSB, ACTA2) markers and TRP channels (TRPV2, TRPV4, TRPV6, TRPC1, TRPC4, TRPC6, TRPM4 and TRPM7) in primary and metastatic tumor biopsies. N = 22 p.s. Results are shown as mean relative expression to the geometric mean of housekeeping genes HPRT, PGK, ACTB and GAPDH. B , D Relative expression of marker genes and TRP channels displaying a significant association with either the primary or metastatic nature of the tumour biopsy. * p < 0.05 ** p < 0.01, *** p < 0.001 using univariate linear regression with mRNA expression (ΔCT) as dependent variable and biopsy status as independent variable. N = 22 p.s. E Pearson correlation coefficients between TRP channels and epithelial (CDH1, EPCAM, KRT18, MMP7) and mesenchymal (CDH2, ACTA2, MMP2, MMP9, ZEB1 and CTSB) marker genes in metastatic EC biopsies. Red indicates a positive correlation, while blue indicates a negative correlation. * p < 0.05 ** p < 0.01. N = 22 p.s

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-human E-cadherin (1/30, Abcam), rabbit monoclonal anti-human vimentin (1/100, Abcam), mouse monoclonal anti-human cytokeratin (1/100 Agilent), mouse monoclonal anti-human MMP2 (1/100, Abcam), and mouse monoclonal anti-human α-smooth muscle actin (1/100, Agilent).

Techniques: Expressing, Marker

Journal: Cellular and Molecular Life Sciences

Article Title: TRP channel expression correlates with the epithelial–mesenchymal transition and high-risk endometrial carcinoma

doi: 10.1007/s00018-021-04023-1

Figure Lengend Snippet: Relationship between marker gene expression and clinical parameters: A , B Heat maps of mRNA expression of epithelial (CDH1, KRT18, EPCAM, MMP7) and mesenchymal (MMP2, MMP9, CTSB, ACTA2) markers between different clinical parameters (FIGO stage, grade, histology, molecular class, steroid receptor expression, myometrial invasion, LVI and recurrence) in primary EC biopsies. Results are shown as mean relative expression to the geometric mean of housekeeping genes HPRT, PGK, ACTB and GAPDH. N = 54 p.s. C Mesenchymal/epithelial ratio between different clinical parameters shown as mean ± 95% CI. Colours indicate ratio’s < 1 (blue) and > 1 (red). The ratio was constructed for each biopsy using median expression values. D – H Relative expression of marker genes displaying significant associations with certain clinical parameters: stage (compared to Ia) ( D ), grade ( E ), histology (compared to endometrioid) ( F ), myometrial invasion ( G ) and recurrence ( H ). Data are represented as mean ± SEM. * p < 0.05 ** p < 0.01 using univariate linear regression with mRNA expression (ΔCT) as dependent variable and clinical parameters as independent variable. N = 54 p.s

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-human E-cadherin (1/30, Abcam), rabbit monoclonal anti-human vimentin (1/100, Abcam), mouse monoclonal anti-human cytokeratin (1/100 Agilent), mouse monoclonal anti-human MMP2 (1/100, Abcam), and mouse monoclonal anti-human α-smooth muscle actin (1/100, Agilent).

Techniques: Marker, Gene Expression, Expressing, Construct

Journal: Cellular and Molecular Life Sciences

Article Title: TRP channel expression correlates with the epithelial–mesenchymal transition and high-risk endometrial carcinoma

doi: 10.1007/s00018-021-04023-1

Figure Lengend Snippet: Characterization of EMT status of EC cells: A Brightfield pictures (magnification: 10×) of different EC cells EM018a, EM012, EM033, EM046. Scale bar = 100 µm. B Heat map of mRNA expression of epithelial (CDH1, KRT18, EPCAM, MMP7) and mesenchymal (MMP2, MMP9, ZEB1, CTSB, ACTA2, CDH2 and VIM) markers genes. Data are represented as relative expression to housekeeping genes HPRT and PGK. Stage and histology of the corresponding cells is indicated on the figure. C – F Immunofluorescence stainings (magnification: 20×) of the epithelial markers E-cadherin ( C ) and cytokeratin ( D ) and mesenchymal markers MMP2 ( E ) and vimentin ( F ) in EC cells EM018a, EM012, EM033, EM046. Scale bar = 50 µm.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-human E-cadherin (1/30, Abcam), rabbit monoclonal anti-human vimentin (1/100, Abcam), mouse monoclonal anti-human cytokeratin (1/100 Agilent), mouse monoclonal anti-human MMP2 (1/100, Abcam), and mouse monoclonal anti-human α-smooth muscle actin (1/100, Agilent).

Techniques: Expressing, Immunofluorescence

Journal: Oncology letters

Article Title: The combined use of EphA2/MMP-2 expression and MRI findings contributes to the determination of cerebral glioma grade.

doi: 10.3892/ol.2019.10912

Figure Lengend Snippet: Figure 1. Immunohistochemical staining of glioma tissues. (A) MMP‑2 and (B) EphA2 expression was identified by immunofluorescence staining in the cytoplasm of glioma cells. Positive cells were stained brown. Percentages of stained cells are presented in the bar graphs. Data represent the mean ± standard deviation (n=5). ***P<0.001. Scale bar, 30 µm. EphA2, ephrin type‑A receptor 2; MMP‑2, metalloproteinase 2.

Article Snippet: One slide was routinely stained with hematoxylin and eosin in 25 ̊C for 1‐5 min. After blocking in BSA solution 37 ̊C for 30 min (cat. no. P0260; Beyotime Institute of Biotechnology), the other slide was used for IHC staining using mouse anti-human MMP-2 and mouse anti-human EphA2 (Boster cat. no. M00286 and BM0833, both 1:200) monoclonal antibodies detected by streptavidin-peroxidase or 3'-diaminobenzidine visualization kits (OriGene Technologies, Inc.).

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence, Standard Deviation

Journal: Oncology letters

Article Title: The combined use of EphA2/MMP-2 expression and MRI findings contributes to the determination of cerebral glioma grade.

doi: 10.3892/ol.2019.10912

Figure Lengend Snippet: Figure 3. Pearson's correlation analysis of the magnetic resonance imaging parameters and immunohistochemistry results. Scatterplots presenting the correla- tion between (A) MMP‑2 and (B) EphA2 positivity with the EI; (C) MMP‑2 and (D) EphA2 positivity with the EP; and (E) MMP‑2 and (F) EphA2 positivity with maximum tumor diameter. EI, edema index; EP, enhancement percentage; EphA2, ephrin type‑A receptor 2; MMP‑2, metalloproteinase 2.

Article Snippet: One slide was routinely stained with hematoxylin and eosin in 25 ̊C for 1‐5 min. After blocking in BSA solution 37 ̊C for 30 min (cat. no. P0260; Beyotime Institute of Biotechnology), the other slide was used for IHC staining using mouse anti-human MMP-2 and mouse anti-human EphA2 (Boster cat. no. M00286 and BM0833, both 1:200) monoclonal antibodies detected by streptavidin-peroxidase or 3'-diaminobenzidine visualization kits (OriGene Technologies, Inc.).

Techniques: Magnetic Resonance Imaging, Immunohistochemistry